The PSpice for TI design and simulation environment allows you to simulate complex mixed-signal designs with its built-in library. Available at no cost, PSpice for TI includes one of the largest model libraries in the industry, spanning our analog and power portfolio, as well as select analog behavioral models. This full-featured, design and simulation suite uses an analog analysis engine from Cadence®. PSpice® for TI is a design and simulation environment that helps evaluate functionality of analog circuits.
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Our results show that the trends of resting and exercise-induced wall shear stress waveforms, especially the maximal, minimal and mean wall shear stress as well as oscillatory shear index, generated by the parallel-plate flow chamber system are similar to those acquired from the common carotid artery. To validate the feasibility of this system, human umbilical vein endothelial cells (HUVECs) line were cultured within the parallel-plate flow chamber under abovementioned two types of wall shear stress waveforms and the intracellular actin microfilaments and nitric oxide (NO) production level were evaluated using fluorescence microscope. According to the above in vivo wall shear stress waveforms, we designed and fabricated a parallel-plate flow chamber system with appropriate components based on a lumped parameter hemodynamics model. The in vivo wall shear stress waveforms from the common carotid artery of a healthy volunteer in resting and immediately after 30Â min acute aerobic cycling exercise were first calculated by measuring the inner diameter and the center-line blood flow velocity with a color Doppler ultrasound. To confirm the key role of wall shear stress induced by exercise on endothelial cells, and to understand how wall shear stress affects the structure and the function of endothelial cells, it is crucial to design and fabricate an in vitro multi-component parallel-plate flow chamber system which can closely replicate exercise-induced wall shear stress waveforms in artery. In vivo studies have demonstrated that reasonable exercise training can improve endothelial function. Wang, Yan-Xia Xiang, Cheng Liu, Bo Zhu, Yong Luan, Yong Liu, Shu-Tian Qin, Kai-Rong A multi-component parallel-plate flow chamber system for studying the effect of exercise-induced wall shear stress on endothelial cells. |
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